Fig. 1
From: Characterizing glucokinase variant mechanisms using a multiplexed abundance assay
Measuring glucokinase variant abundance by DHFR-PCA. A Overview of the Dihydrofolate Reductase Protein-Fragment Complementation Assay (DHFR-PCA). B Low-throughput test of the DHFR-PCA. Selected glucokinase variants expressed in wild-type yeast cells were grown on medium without (DMSO) or with methotrexate (MTX) to assess their impact on cellular protein abundance. The vector control did not contain the DHFR-PCA sequences. C Overview of the multiplexed assay for glucokinase variant abundance