Fig. 4
From: From beer to breadboards: yeast as a force for biological innovation
The barcode fusion genetics (BFG) approach combines two barcoded populations into individual cells to increase screening throughput [127]. The BFG technology enables phenotypic analysis of a heterogeneous pool of millions of yeast strains, each having two engineered loci or genes of interest. In BFG, a doubly engineered cell pool is prepared using either (1) mating of two haploid populations (maroon and orange strains in the left panel), (2) plasmid transformation with a barcoded gene bank, or (3) microencapsulation of two yeast cells into a single emulsion droplet. Once segregated, the transfer of a DNA barcode flanked by site-specific recombination (based upon Cre-Lox recombination) sequences is initiated to yield a single, doubly barcoded molecule (center panel). Strain abundances can then be quantified by amplification and deep sequencing of fused barcodes (right panel)