Fig. 5

Identification of RBM5 downstream target genes in AML. a The transcriptome analysis in three groups of MOLM13 cells, including the CTRL group (control cells with NT sgRNA treated), RBM5 KO group (RBM5 knockout cells with RBM5-sg1 treated), and RBM5 OE group (RBM5 overexpressed cells by lentiviral RBM5-P2A-venus vector). Each group was performed with three biological replicates. Integrated scatter plot analysis for all genes from the RNA-seq dataset by comparing RBM5 OE vs. CTRL and RBM5 KO vs. CTRL in MOLM13 cells (left panel) (n = 13,178). The graph on the right panel shows the significant genes (n = 154). False discovery rate [FDR] < 0.05. b Heat map showing filtered genes whose expression is significantly differentially expressed after RBM5 loss in three groups of MOLM13 cells, including the CTRL, RBM5 KO, and RBM5 OE. c Leading edge plot from gene enrichment analysis (GSEA) showing the enrichment of indicated pathway for genes in RBM5 OE compared to control. Enrichment scores, P-values, and FDR (false-discovery rate) are calculated as shown in the plot. d Leading edge plot from gene enrichment analysis (GSEA) showing the enrichment of indicated pathway for the decreased genes in RBM5 KO cells relative to control. Enrichment scores, P-values, and FDR (false-discovery rate) are calculated as shown in the plot. e Venn diagram showing the overlap between genes differentially expressed and genes undergoing altered splicing events after RBM5 knockout (from RNA-seq). f Sashimi plots the example gene PWWP3A and FLT3 in RBM5 KO and CTRL group in MOLM13 cells