Fig. 5

The CRISPRi V2 LGR library identifies more bonafide hits in a 100-fold cell coverage K562 dasatinib survival screen. A Schematic of the dasatinib survival screen performed using the LGR and legacy libraries at 100-fold cell coverage. B Precision-recall analysis of the essential genes identified in each library using BAGEL2 [23, 24] to measure screening quality. The area under the curve (AUC) for each library were as followed: LGR 0.936 and legacy 0.911. C Comparison of essential gene hits (T₀ versus DMSO samples) identified in the LGR (right) versus the legacy (left) libraries. D Comparison of dasatinib treatment hits (dasatinib treatment versus DMSO control samples) identified in the LGR (right) versus the legacy (left) libraries. E MAGeCK-VISPR was used to determine the number of gene hits identified in each library at false discovery rates (FDR) ranging from 0.25 to 0.001. Hits were categorized as positive (increased survival) or negative (decreased survival)