Fig. 3

The optimized LGR library performs similarly to the existing legacy library, even at lower cell coverage. A Schematic of the CRISPRi V2 survival screen in K562 cells performed with the LGR library at 100- or 1000-fold cell coverage. B Comparison of the 1000-fold cell coverage screen performed by Horlbeck et. al (2016) [18] using the CRISPRi V2 legacy library (left) versus the 1000-fold (middle) and 100-fold (right) cell coverage screens performed using the CRISPRi V2 LGR library. C Precision-recall analysis of the essential genes identified in each library using BAGEL2 [25, 26] to measure screening quality between the legacy library and the LGR library. The area under the curve (AUC) for each library were as follows: legacy (Horlbeck et. al 2016) [18] 0.920, LGR 1000-fold 0.937, and LGR 100-fold 0.949. D A diagram illustrating the amount of overlap in essential genes identified in each screen. E A point comparison of the phenotype score of overlapping hits between the CRISPRi V2 LGR 100 and 1000-fold screens