Fig. 5

Comparison between mouse and human GCLiPP reveals principles of shared post-transcriptional regulation. A Schematic illustration of 3′ UTR alignment and biochemically shared GCLiPP peak calling. B Distribution of conservation across 100 vertebrates (PhyloP score) of regions in the human genome. Blue indicates biochemically shared peaks and gray indicates the 3′ UTRs of the transcripts that those peaks are contained within. For both peaks within ARRB2 and USP25, their matched conservation of peak and UTR are indicated by connected vertical lines. C Human and mouse normalized GCLiPP density and conservation (PhyloP) across aligned nucleotides of the indicated 3′ UTRs. Biochemically shared peaks of GCLiPP read density are indicated in pink. D HOMER called motifs enriched in biochemically shared peaks. Percentages indicate the frequency of occurrence of the indicated motif in biochemically shared peaks versus non-shared background peaks. P-value indicates HOMER calculated p-value of enrichment. E Metascape called biological enrichment categories of genes containing biochemically shared peaks. The background set was all genes that contained peaks in both mouse and human GCLiPP datasets that did not contain a shared peak