Fig. 3

Activation-induced changes in RBP binding. A Correlation between activation-induced changes in RBP expression and protein occupancy at corresponding binding sites in Jurkat cells. Mean differential binding intensities were calculated for sets of GCLiPP peaks defined by their overlap with specific RBP binding in ENCODE eCLIP datasets. Dots represent individual RBPs, and lines show the concordance between the first principal component (blue) describing variation within these data with the line of unity (red). B Enrichment of RBP consensus binding motifs centered within regions with increased GCLiPP signal in activated vs. resting Jurkat cells. Genomically encoded motifs recognized by 4 PABPC family members were enriched in regions differentially bound in resting, but not activated Jurkat cells. C–E Cumulative distribution function (CDF) plots depicting the distance from the translation stop codon for the top 10% of differentially bound regions in activated compared to resting Jurkat cells filtered for those containing a canonical PAS (C), genomic templated PABPC binding site (D), or any GCLiPP peak (E)