Fig. 5
From: Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies
PML knockdown inhibits differentiation in H3.3 WT (K27M KO) glioma cells. A Immunofluorescence of K27M (green) and PML (red) in DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M pediatric glioma cells (clone #1) with and without doxycycline-induced PML knockdown. Scale bar 2 µM. B Immunofluorescence staining of GFAP glial marker (green) and β-tubulin (red) of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells (clone #1) with and without PML knockdown, over 7 and 14 days of culture in differentiation media. C Western blots of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells overexpressing myc/flag tagged PML (denoted + PML) using anti-myc and actin antibodies. D Immunofluorescence staining of GFAP glial marker (green) and β-tubulin (red) of DIPG XIII H3.3 WT (K27M KO) and H3.3 K27M glioma cells with and without PML overexpression, over 7 and 14 days of culture in differentiation media