Fig. 1

Somatic point mutations are detectable in spatial transcriptomics data. A–C H&E stains are shown for cutaneous squamous cell carcinomas from patients 4 and 6 of Ji et al. Cell (2020). The sections underwent histopathologic assessment, and the areas of cutaneous squamous cell carcinoma (cSCC), epidermal hyperplasia, or actinic keratosis are circled as shown. DNA sequencing was performed on these tumors to call somatic mutations. Spots from the spatial transcriptomics arrays are colored based on the presence or absence of sequencing reads mapping to the mutant or reference alleles over somatic mutation sites. Mutant spots were enriched in tumor areas, though some mutant spots were observed in normal tissue (epidermal hyperplasia) or pre-malignant tissue (actinic keratosis) in patient 4, as shown in C