Fig. 7

Inhibition of MLL1 by OICR-9429 repressed CRC and regulated tumor immunity. A The sketch for CRC animal model treated with OICR-9429. B The representative image for colons with/without OICR-9429. Tumors were measured, and the total tumor number and size for each animal was plotted in a line for the DMSO and OICR-9429 groups. C The histogram showing the tumor size distribution. D Representative confocal images and quantitation of tumor stained with Ki67 in AOM-DSS induced CRC tumors. n = 30 FoV. E–G Representative confocal micrographs of CD3⁺CD4⁺ T helper (E), CD3⁺CD8⁺ T cytotoxic lymphocytes (F) and F4/80⁺CD206⁺ M2 type of TAM (tumor-associated macrophage) (G). H-J Quantification of CD3⁺CD4⁺ T helper (H), CD3⁺CD8⁺ T cytotoxic lymphocytes (I), and F4/80⁺CD206⁺ M2 TAM (J). n = 20 FoV. K Representative confocal micrographs and quantification of NK cell marker NKp46 in AOM-DSS induced CRC tumors. n = 20 FoV. * means p value < 0.05, ** for p value < 0.01. Data are represented as mean ± SEM. p-values are calculated by two-tailed unpaired t test