Fig. 6
From: Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancer
JUN regulates m3Es and interacts with MLL1. A DNA motifs enriched within nucleosome-free regions (NFRs) of gain Vm3Es determined by HOMER motif analysis. B Bar plot showing the relative mRNA level of the proximal genes for RPS6KA5 and CBX8 enhancers in JUN-KD HCT116 cell line. C Western blot of the proximal genes for RPS6KA5 and CBX8 m3Es in HCT116 with JUN knockdown. D Capture-ChIP qPCR showing the enrichment of JUN at m3Es in JUN-overexpressed HCT116. E & F ChIP-qPCR assessing H3K27ac and H3K4me3 level at RPS6KA5, CBX8, CCND1 m3Es in JUN-KD HCT116 cell line. G Western blot of MLL1 or JUN after co-immunoprecipitation (co-IP) with indicated antibodies in HCT116. * means p value < 0.05, ** for p value < 0.01. Data are represented as mean ± SD or SEM. p-values are calculated by two-tailed unpaired t test