Fig. 5
From: Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancer
MLL1/KMT2A regulates H3K4me3 on m3Es. A-C Bar plot showing the relative mRNA levels of the proximal genes for CBX8, RPS6KA5, CCND1 m3Es in KMT2A-KD HCT116 cells. D Western blot detection of the protein level of the proximal genes for CBX8, RPS6KA5, CCND1 m3Es in KMT2A-KD HCT116 cells. E–G ChIP-qPCR assessing H3K27ac and H3K4me3 level at CBX8, RPS6KA5, CCND1 m3Es in KMT2A-KD cell line. H & I Bar plot showing the mRNA levels of the proximal genes and histone modifications of CBX8 m3E in HCT116 after OICR-9429 (10 µM-48 h) treatment. J Western blotting of the proximal genes for CBX8 m3E after OICR-9429 (10 µM, 48 h) treatment. K & L Bar plot showing the mRNA levels of the proximal genes and histone modifications of CBX8 m3E in HCT116 after C646 (10uM-12 h) treatment. M Western blotting of the proximal genes for CBX8 m3E after C646 (10 µM, 12 h) treatment. * means p value < 0.05, ** for p value < 0.01. Data are represented as mean ± SD or SEM. p-values are calculated by two-tailed unpaired t test