Fig. 3
From: Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancer
Validation of the identified Vm3Es and their roles in transcription regulation. A ChIP-qPCR showing H3K27ac and H3K4me3 levels at m3Es in HCT116. B Native ChIP-qPCR showing the H3K4me3 level at m3Es in HCT116. C-E Bar plot showing the relative mRNA level of the proximal genes for m3Es in control and sgRNA groups (n = 3). sgRNA targeting EGFP was used as the control in the following experiments. F–H ChIP-qPCR showing H3K27ac and H3K4me3 level at CBX8, RPS6KA5, TNFSF10 enhancer loci in control and sgRNA groups. * means p value < 0.05, ** for p value < 0.01. Data are represented as mean ± SD or SEM. p-values are calculated by two-tailed unpaired t test