Fig. 1
From: Fake IDs? Widespread misannotation of DNA transposons as a general transcription factor
GTF2IRD2/2A is used to erroneously misannotate DNA TEs. A The phylogenetic relationship of sequences annotated as GTF2IRD2/2A from bony fishes, reptilian, amphibian, and mammalian genomes from NCBI. Bony fish are coloured green, reptiles are orange, amphibians are pink, and mammals are blue. Branches coloured in red are hAT-6 transposons. The Hermes transposase was used as an outgroup. Support values under 60 are shown at nodes. For the full tree and support values, see Additional file 4. B Boundary of non-transposon and Charlie8-like domain of a multiple sequence alignment (MSA) of mammalian GTF2IRD2/2A to hAT-6 transposons and a TE sequence misannotated as GTF2IRD2-like. C Full schematic MSA of the selected sequences. The length of sequences in amino acids is shown on the x-axis from the 5′ to 3′ direction. The Charlie8-like domain of mammalian GTF2IRD2/2A is highlighted in red text and red shading. ‘*’ denotes a single, fully conserved amino acid within the alignment