Fig. 3

Candidate modulators identified from the screen regulate hepatocyte marker expression. a Schematic illustration of the strategy to filter unperturbed cells for expression analysis. b The representative distribution curve of perturbation probability of cells with sgRNA targeting NT-2 (non-targeting) and Foxs1-1 sgRNA. c, d Violin plot showing the relative effects of each perturbation from module P2 (c) or module P4 (d) on selected known hepatic markers compared to NT controls. Color filling indicates a P-value less than 0.05 (Wilcoxon rank sum test). e, f Perturbation score of candidate modulators in P2 (e) and P4 (f) groups using hepatocyte regulator score calculated with expression change of the nine hepatocyte markers used in c and d