Phytochromes transmit photoperiod information via the evening complex in Brachypodium

Table 1 ELF3 interacts with other evening complex proteins and the light signaling network in Brachypodium

Gene ID	Protein/homolog protein	Molecular weight	Exclusive unique peptide count/percent coverage^a
Gene ID	Protein/homolog protein	Molecular weight	rep1	rep2
Bradi1g64360	phyB	129 kD	32/39%	24/30%
Bradi1g08400	phyC	126 kD	2/2.8%	2/2.9%
Bradi2g00992	BED ZINC FINGER AND HAT DIMERIZATION DOMAIN-CONTAINING PROTEIN	82 kD	13/26%	11/25%
Bradi2g09080	TOPLESS	125 kD	18/22%	7/6.6%
Bradi3g16250	TOPLESS	125 kD	18/20%	10/11%
Bradi3g57667	SPA1	77 kD	13/27%	8/18%
Bradi2g46850	PCH1^b	83 kD	10/22%	5/14%
Bradi4g07110	MYB transcription factor	110 kD	15/25%	4/5%
Bradi3g03317	Splicing factor	136 kD	16/17%	3/3.5%
Bradi1g24100	TIME FOR COFFEE	171 kD	8/10%	9/9.3%
Bradi2g62067	LUX	27 kD	3/31%	2/20%
Bradi2g48657	SPA3	87 kD	12/22%	7/14%
Bradi4g13227	ELF4	13 kD	3/33%	4/34%
Bradi1g05950	MLK1/3/4 or PPK2/3/1	79 kD	10/23%	4/9.6%
Bradi1g05950	FVE	50 kD	9/41%	4/16%
Bradi3g19927	KNOX domain-containing protein	33 kD	4/11%	4/11%

Curated list of proteins co-purified with BdELF3-GFP-FLAG which were specifically identified from affinity purification coupled with mass spectrometry (AP-MS) analyses using 45-day-old plants (old, 2 reps) or 21- day-old plants (young, 1 rep) harvested at ZT0 in dark (14 h light at 24 °C:10 h dark at 18 °C). Negative control YFP-HFC does not detect any peptides of those proteins
^aAll listed proteins match 99% protein threshold, minimum number peptides of 2, peptide threshold as 95%, and not detected in the YFP-HFC control
^bBrachy PCH1 may has F-box domain as peptide detected in the C-terminus

ISSN: 1474-760X