Fig. 2
From: Phytochromes transmit photoperiod information via the evening complex in Brachypodium
BdELF3 interacts with BdLUX. A BdELF3 and BdLUX interact in the yeast 2-hybrid system. Yeast transformed with BdLUX fused to the GAL4 DNA binding domain (BD) and BdELF3 with the activation domain (AD) are able to grow on –His media indicating interaction between the proteins. This interaction is dependent on the proteins, as empty vector controls (---) do not support growth on –His. B Split luciferase (LUC) complementation assay shows interaction between BdELF3 and BdLUX in vivo. Tobacco leaves have been infected with BdLUX fused to the C-terminal domain of LUC and BdELF3 with the N-terminal domain of LUC. This interaction is specific as empty vector controls (EV) emit no Luciferin signal