Fig. 4

Automatic technology corrections. A Showing atlas metacells used to represent query metacells when projecting 10x-v3 blood data on the 10x-v2 BM atlas (as shown in Fig. 1). B Shown are distributions of observed vs, projected expression R², with and without automatic gene correction. C Examples of corrected genes. Correction factor (constant in log-scale) is shown as a red line. D MCProj applies auto-gene correction when the correlation between observed and projected expression overall all metacell is high, and the correction factor is of a large magnitude. Shown here are correlation and optimal correction factor values for all genes when applying MCProj as in A. Red dots represent genes that were selected for correction. E R² values of metacell genes successfully projected on the batch-punctuated atlas (Fig. 2), when varying the strength of a synthetic technology bias applied to the genes of the batch, where the full strength is identical to the one computed in Fig. 4C. F Similar to E, but when varying the fraction of genes that are synthetically modified by a multiplicative factor