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Table 2 Performance comparison of the different clustering methods on the dataset\(S_1 \sim S_6\). Here, Identity is the parameter of clustering tools, AMI is the abbreviation of adjusted mutual information, FMI is the abbreviation of Fowlkes-Mallows Index, ACC is the abbreviation of accuracy, HOMO is the abbreviation of homogeneity, and COMP is the abbreviation of completeness

From: HycDemux: a hybrid unsupervised approach for accurate barcoded sample demultiplexing in nanopore sequencing

Dataset

Tool

Identity (%)

AMI (%)

FMI (%)

ACC (%)

HOMO (%)

COMP (%)

Time (min:sec)

S1

MeShClust

80.0

58.17

34.51

42.16

64.46

54.57

1:07.81

85.0

58.31

36.65

44.31

67.53

53.91

1:28.03

90.0

62.69

40.18

36.35

86.60

51.69

0:58.17

95.0

56.66

33.69

24.86

94.59

45.62

1:00.59

CD-HIT

80.0

77.63

66.28

57.32

100.00

64.61

0:01.03

85.0

70.51

59.47

49.78

100.00

57.06

0:01.60

90.0

58.73

45.50

36.92

100.00

47.41

0:02.67

95.0

41.30

26.92

18.71

100.00

37.89

0:04.00

UCLUST

80.0

61.74

47.37

37.97

100.00

49.06

0:00.59

85.0

54.53

38.50

28.34

100.00

44.09

0:00.80

90.0

44.19

27.02

17.64

100.00

38.64

0:01.09

95.0

32.67

18.10

11.48

100.00

34.33

0:01.44

HycDemux

-

97.73

97.40

96.75

99.99

95.59

0:10.95

S2

MeShClust

80.0

67.10

50.96

57.66

70.45

64.98

0:55.28

85.0

67.63

52.12

52.67

83.91

58.61

1:12.78

90.0

61.96

50.08

41.15

92.98

50.83

1:00.49

95.0

59.67

49.57

44.31

96.26

49.03

1:56.98

CD-HIT

80.0

81.89

71.30

65.57

100.00

70.02

0:01.40

85.0

73.60

60.10

52.22

100.00

60.09

0:02.36

90.0

60.24

42.76

33.59

100.00

48.08

0:04.63

95.0

40.52

24.03

16.17

100.00

37.37

0:07.25

UCLUST

80.0

63.74

45.82

35.77

100.00

50.27

0:00.96

85.0

53.73

32.44

22.27

100.00

43.06

0:01.35

90.0

40.88

20.52

13.63

100.00

36.89

0:01.91

95.0

26.29

12.46

7.34

100.00

32.38

0:02.44

HycDemux

-

99.89

99.91

99.91

100.00

95.59

0:13.12

S3

MeShClust

80.0

85.06

83.40

86.24

93.44

79.06

1:33.12

85.0

79.99

81.27

79.19

96.50

70.48

1:58.35

90.0

66.37

65.43

61.96

94.12

56.85

2:01.84

95.0

57.65

58.07

45.25

99.47

48.83

1:07.26

CD-HIT

80.0

88.70

83.32

78.95

100.00

79.99

0:01.65

85.0

81.22

74.83

67.45

100.00

69.48

0:03.20

90.0

67.86

60.14

52.22

100.00

55.24

0:06.92

95.0

44.53

30.26

21.45

100.00

39.50

0:04.00

UCLUST

80.0

68.75

54.50

44.48

100.00

55.08

0:01.29

85.0

58.44

44.18

32.51

100.00

46.91

0:01.79

90.0

44.36

26.50

18.00

100.00

38.76

0:02.46

95.0

26.93

14.09

8.60

100.00

32.67

0:01.44

HycDemux

-

97.78

96.90

95.39

100.00

95.67

0:19.34

S4

MeShClust

80.0

54.00

24.31

32.06

65.57

50.39

0:47.93

85.0

49.58

17.13

27.23

59.03

47.54

0:57.78

90.0

55.31

28.55

23.48

87.42

49.55

1:01.96

95.0

50.60

26.03

19.31

92.11

47.40

1:04.56

CD-HIT

80.0

78.29

59.75

50.20

99.99

67.34

0:01.28

85.0

69.95

50.26

40.07

100.00

59.80

0:01.89

90.0

56.29

36.47

26.12

100.00

51.10

0:03.31

95.0

35.86

20.55

12.87

100.00

43.17

0:04.77

UCLUST

80.0

61.21

39.56

29.19

100.00

53.59

0:00.79

85.0

53.42

32.61

22.30

100.00

49.51

0:01.03

90.0

40.48

21.84

13.65

100.00

44.40

0:01.46

95.0

27.05

14.30

8.36

100.00

40.78

0:01.85

HycDemux

-

98.42

97.03

96.28

99.73

97.18

0:19.96

S5

MeShClust

80.0

68.39

47.68

52.91

82.86

62.65

1:03.37

85.0

62.40

36.39

46.49

82.04

57.61

1:43.73

90.0

54.90

24.28

33.06

73.30

52.13

1:55.66

95.0

53.89

24.53

31.02

82.26

51.23

1:06.23

CD-HIT

80.0

85.39

71.10

64.08

100.00

75.94

0:01.66

85.0

77.87

60.19

51.56

100.00

67.23

0:02.76

90.0

65.62

48.89

39.54

100.00

57.13

0:04.93

95.0

45.10

28.76

20.16

100.00

46.40

0:07.29

UCLUST

80.0

70.56

55.00

43.23

100.00

60.60

0:01.13

85.0

60.71

44.25

32.51

100.00

53.79

0:01.56

90.0

46.99

30.48

20.19

100.00

47.03

0:02.12

95.0

30.91

18.12

10.80

100.00

41.81

0:02.50

HycDemux

-

98.77

97.43

95.90

99.99

97.61

0:29.50

S6

MeShClust

80.0

87.66

79.22

83.36

93.81

83.40

0:49.57

85.0

83.44

76.77

77.05

95.24

76.69

1:01.72

90.0

76.70

71.47

68.35

96.18

68.72

1:03.75

95.0

70.43

64.56

56.08

98.07

62.35

1:05.74

CD-HIT

80.0

92.46

86.29

82.23

100.00

86.44

0:01.58

85.0

86.30

77.45

72.41

100.00

77.51

0:02.80

90.0

74.23

61.01

52.30

100.00

64.25

0:05.54

95.0

52.12

32.98

24.42

100.00

49.34

0:09.23

UCLUST

80.0

78.99

67.27

57.71

100.00

68.73

0:01.27

85.0

69.14

54.09

44.34

100.00

59.75

0:01.73

90.0

53.36

34.91

25.64

100.00

49.81

0:02.39

95.0

31.74

15.71

9.68

100.00

41.86

0:03.07

HycDemux

-

99.40

99.11

98.93

100.00

98.82

0:33.77