Fig. 5
From: Coupling of co-transcriptional splicing and 3’ end Pol II pausing during termination in Arabidopsis
Inhibition of splicing reduces the extent of 3’ end Pol II pause. a Metagene plots showing Pol II profiles of paused and non-paused genes in samples treated with DMSO (the control) or herboxidiene (splicing inhibitor). Only the genes that are active (TPM ≥ 1 based on the Ser2P Pol II pNET-seq data) in both DMSO and herboxidiene-treated samples are used for this analysis. Paused and non-paused genes were defined based on the 3’PI of the control sample treated with DMSO. b Box plot comparison of the 3’PI between DMSO-treated and herboxidiene-treated samples. 3’PI of genes that display 3’ end pause both in DMSO- and herboxidiene-treated samples are shown. p value was calculated based on a paired Wilcoxon test. c The relationship between 3’PI and exon numbers in samples treated with DMSO (left) or herboxidiene (right). The Spearman correlation coefficient is indicated at the top of the plot. Gene numbers of each group are indicated above the boxes. d Gene examples of which the herboxidiene treatment reduces the 3’ end pause. Genome browser tracks indicate the normalized counts from pNET-seq data and total RNA-seq data. Bar charts below the figure demonstrate the qPCR validation of altered splicing efficiency. Primer locations are labelled on the top (spliced primer) or the bottom (unspliced primer) of the gene structure. Data are presented as mean ± SD (n = 3). Asterisk indicates a significant difference based on a two-tailed t-test (* p < 0.05, ** p < 0.01, *** p < 0.001)