Fig. 2

Pol II pause at gene 3’end is not mediated by XRN3. a Venn diagram showing the overlap among paused genes in Col-0, paused genes in xrn3, and genes that display increased readthrough in xrn3 compared to Col-0. b Diagram showing the definition of readthrough index (RTI). c Metagene plot showing Pol II profiles at 3’ end of genes with increased readthrough in xrn3 compared to Col-0. d Typical examples from the gene group as shown in c. Genome browser tracks showing the normalized counts from pNET-seq data (1 nt resolution) and chromatin-bound RNA-seq data. The gray dashed box highlights the region with readthrough. The black arrow indicates the zoom-in view of the region with readthrough. e Box plot demonstrates the distribution of 3’PI in Col-0 and xrn3 at genes with increased readthrough in xrn3 compared to Col-0. The line in the box plot indicates the median, box edges represent the first and third quartiles, and the whiskers extend to the farthest data points within 1.5 × interquartile range outside box edges. p value was calculated based on a paired Wilcoxon test