Fig. 7

Injection order contributes to proteome differences. A Experimental study design. B Proteins identified in the Quartet samples using three different injection orders (left) and Venn diagram of the identified proteins (right). C Pearson’s correlation coefficients matrix representing the correlation coefficients between different mass spectrometry runs generated by three different injection orders. D Principal component analysis and signal-to-noise ratio (SNR) scores for datasets produced using different injection orders and the integrated dataset. E Four-dimensional visualization map. RI: random injection order (5678–5678-5678); CI1: continuous injection order 1 (5678–5678-5678); CI2: continuous injection order 2 (555–666-777–888)