Fig. 5

Underestimated MAP expression in healthy tissues. a–h Published human colorectal cancer (CRC) TAAs, mutated TAs, ERE-derived TSAs, proteasomal splicing peptides, and Epstein–Barr virus (EBV) MAPs were searched with BamQuery in the GTEx tissues (n = 12–50 / tissue), mTECs (n = 11), and DCs (n = 19) bam files in unstranded mode with genome version GRCh38.p13, gene set annotations release v38_104 and dbSNP release 155 (except for the search for mutated TAs (d) where dbSNP was not considered, dbSNP = 0). a Heatmap of average RNA expression of published CRC TAAs in indicated tissues. Boxes in which a peptide has an average rphm > 8.55 are highlighted in black. b Heatmap of average RNA expression of the CRC mutated TA RYLAVAAVF and its wild-type RYLTVAAVF in indicated tissues. c Percentage of the most likely biotype attributed by BamQuery to published fusions, junctions, and SNVs-derived TAs. d Heatmap of average RNA expression of published mutated TAs (n = 23) in indicated tissues. The number of genomic locations expressed is presented on the left. e Number of genomic locations at which the expression of the ERE TSAs was assessed by BamQuery vs. the original study. Light blue dots represent each assessed MAP, and the orange triangle represents the average. f Heatmap of average RNA expression of the EREs-derived TSAs in mTECs, normal breast tissues from GTEx (n = 50), and triple-negative breast cancer samples from TCGA (n = 158). g Heatmap of average RNA expression of published proteasomal splicing MAPs (n = 99) in indicated tissues. The number of genomic locations expressed is presented on the left. h Heatmap of average RNA expression of EBV MAPs in indicated tissues