Fig. 3

ePPEplus for improvement of prime editing efficiency. a Representation of the PPE, ePPE, ePPE-V223A, ePPEmax*, and ePPEplus constructs. b Comparison of the prime editing efficiencies and byproduct efficiencies of five different prime editors (PPE, ePPE, ePPE-V223A, ePPEmax*, and ePPEplus) at 12 target sites in wheat protoplasts. Frequencies (mean ± s.e.m.) were calculated from three independent experiments (n = 3). c Summary of the fold change in prime editing efficiencies for PPE, ePPE-V223A, ePPEmax*, and ePPEplus compared to ePPE. Values were calculated from the data presented in b. The editing frequencies using ePPE for each target were normalized to 1, and the frequencies using PPE and other ePPEs for each target were adjusted accordingly. P values were obtained using the two-tailed Student’s t test: ****P < 0.0001