Fig. 5
ECT2/ECT3/ECT4 stabilize the ABA response-related genes. a Integrative genomics viewer showing the indicated sequencing results on DWA1, DWA2, SDIRIP1, and CPN20 transcripts. The light blue box labeled in each sequencing result indicated the position of the m6A site, the binding sites of ECT2, PAB2, and PAB4. b m6A-IP-qPCR validation of the m6A peaks in DWA1, DWA2, SDIRIP1, and CPN20 under Mock and ABA treatment. Data are presented as means ± SE, n = 2 biological replicates × 2 technical replicates. c FA-RIP-qPCR validation of the binding ability of ECT2 towards DWA1, DWA2, SDIRIP1, and CPN20 in 12-day-old ECT2:ECT2/ect2-1 seedlings under Mock and ABA treatment. Data are presented as means ± SE, n = 3 biological replicates × 2 technical replicates. **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-sided t-test). d Relative mRNA levels of DWA1, DWA2, SDIRIP1, and CPN20 in 7-day-old WT and ect2/3/4 seedlings under Mock and ABA treatment. TUB8 was used as the internal control gene. Data are presented as means ± SE, n = 3 biological replicates × 2 technical replicates. *P < 0.05, **P < 0.01 (two-sided t-test). e The mRNA lifetime of DWA1, DWA2, SDIRIP1, and CPN20 in 7-day-old WT and ect2/3/4 seedlings. TI, transcription inhibition. 18S was used as the internal control gene. Data are presented as means ± SE, n = 3 biological replicates × 2 technical replicates