Fig. 4

IMP1 deficiency inhibits endoderm differentiation. a Flow cytometric analysis of CXCR4-positive cells in wildtype (WT) and IMP1-KO DE cells. The statistical results were shown on the right (n = 3). b Immunofluorescent staining of DE markers (SOX17, FOXA2) and pluripotency markers (NANOG, SOX2) in WT and IMP1-KO DE cells. Quantitative results were shown on the right (n = 5). Scale bar = 50 μm. c RNA levels of representative endoderm genes, mesoderm genes and pluripotency genes in WT and IMP1-KO DE cells determined by RT-qPCR (n = 3). d Scatterplot showing differentially expressed genes in RNA-seq of WT and IMP1-KO DE cells. Upregulated and downregulated genes upon IMP1 knockout were shown in red and blue, respectively. e GO analysis of the downregulated genes in DE cells upon IMP1 knockout. f The overlap of differentially expressed genes upon HIDEN-KO and IMP1-KO. g The correlation of HIDEN-KO and IMP1-KO affected genes