Fig. 3

Olaparib synthetic lethal screens identify BC-risk genes that regulate the DNA repair pathway. A Experimental approach. B B80-T5 cells infected with control or BRCA1-targeting sgRNAs were treated with olaparib for 7 days. C Summary of hits. Known DNA repair genes were annotated based on [33]. D GSEA pathway enrichment analysis of hits (not including background positive control genes). E Validation of selected hits in a singleton experiment using a crystal violet readout. F Enrichment of genes predicted by various computational and statistical methods among the combined hits from all phenotypic screens. INQ_1—high-confidence INQUISIT predictions (genes scored as INQ_1 due to their driver status were downgraded to INQ_2 for this analysis); INQ_2—moderate-confidence INQUISIT predictions; TWAS—identified by transcriptome wide association studies or eQTL studies