Fig. 1

Adipogenic induction elicits sequential waves of transcriptional up- and downregulation. a Assessment of ASC differentiation into adipocytes by Oil Red-O staining of intracellular lipid droplets in undifferentiated ASCs and in day 15 adipocytes. Scale bars, 20 μm. b Time points of data collection for this study. *Re-analyzed published datasets from Ref. [22]. c Principal component analysis of gene expression from 0 to 72 h in each differentiation replicate (% of variance). d Numbers of differentially expressed genes (DE; FDR ≤ 0.05) between two consecutive differentiation time points. e GSEA of differentiating ASCs (0–72-h time course); Hallmark_Adipogenesis. f Unsupervised expression clusters of DE protein-coding genes based on their mean expression z-scores (numbers of genes shown); see Additional file 1, Fig. S2b for all clusters. g Gene ontology terms (PANTHER biological process) enriched in the expression clusters shown in f; Fisher’s exact test adjusted P-values with FDR ≤ 0.05