Table 3 Assembly statistics for the Pt76 chromosome assemblies. Assembly statistics for the Nanopore and HiFi haplotypes. Nanopore haplotype A has a high proportion of collapsed regions which are absent in haplotype B, leading to its smaller size. The HiFi haplotypes are of similar size and have a higher proportion of complete BUSCOs than the Nanopore haplotype A
| Â | Nanopore chromosomes | HiFi-Canu chromosomes | ||||
|---|---|---|---|---|---|---|
| Â | Haplotype A | Haplotype B | Unplaced contigs | Haplotype A | Haplotype B | Unplaced contigs |
Assembly size | 124.430 Mb | 106.954 Mb | 2.351 Mb | 123.9 Mb | 121.6 Mb | 11.0 Mb |
# of scaffolds | 18 | 18 | 46 | 18 | 18 | 362 |
Scaffold N/L50 | 8/7.596 Mb | 8/6.085 Mb | 12/81.914 Kb | 8/7.7 Mb | 8/7.4 Mb | 140/30.2 Kb |
Max scaffold length | 9.699 Mb | 8.736 Mb | 139.988 Kb | 9.5 Mb | 9.3 Mb | 98.9 Kb |
GC content | 46.64% | 46.61% | 44.43% | 46.6% | 46.7% | 42.4% |
Complete BUSCOs (%) | 91.1% | 68.2% | 0.5% | 95.5% | 95.2% | 2.3% |
Duplicated BUSCOs (%) | 2.6% | 2.2% | 0% | 3.7% | 4.2% | 0.1% |
Fragmented BUSCOs (%) | 6.1% | 7.6% | 0.2% | 3.1% | 2.8% | 0.2% |
Predicted genes | 14,601 | 12,549 | 256 | 14,482 | 13,552 | 571 |