Fig. 5
From: Multi-omics analysis dissects the genetic architecture of seed coat content in Brassica napus
Functional characterization of BnaCCRL as a positive regulator of seed lignin biosynthesis in B. napus. a Seeds of CRISPR/Cas9-induced BnaC07.CCRL and BnaA03.CCRL homozygous double mutant lines (L35, L43, L48) and BnaC07.CCRL, BnaA03.CCRL and BnaC05.CCRL homozygous triple mutant line L21. Bar = 1 cm. b the seed coats of L43 and L21. Bar = 1 cm. Determination of thickness of seed coat (c), SCC (d), lignin content (e), and seed oil content (f) in the BnaCCRL double or triple mutant seeds and the control (WT). Values are means ± SD of three biological replicates (n = 6). Student’s t-test was used for statistical analysis between the BnaCCRL lines and WT (*, P < 0.05)