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Fig. 2 | Genome Biology

Fig. 2

From: Using synthetic chromosome controls to evaluate the sequencing of difficult regions within the human genome

Fig. 2

Sequencing performance metrics using sequins. a (left panel) Per-base normalized coverage and absolute sequencing error distributions for different sequencing libraries. (central and right panel) The relative frequency of transitions and transversions mismatch errors. b Average normalized coverage of libraries relative to GC content. c Average normalized coverage of libraries relative to simple repeats. d Erroneous false-positive rate of variant detection (SNVs and indels) relative to allele frequency. a–d Colors represent the different sequencing technologies/preparation methods to generate each of the seven sequenced libraries, such as HiSeq X Ten/PCR-free (purple), HiSeq 2500/PCR-based (red), NextSeq 500/PCR-based (blue), MGISEQ-2000 (yellow), and ONT PromethION (green)

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Genome Biology

ISSN: 1474-760X

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