Fig. 2

Simultaneous silencing LDHA and LDHB reduced lactyation levels and inhibited ocular melanoma tumorigenesis. a Schematic diagram of histone lactylation inhibition methods target. b, c Intracellular lactate levels were measured from OCM1 (b) and CRMM1 (c) cells cultured in different concentrations of 2-DG or oxamate for 24 h by a lactate colorimetric kit. Statistical significance was determined using one-way ANOVA followed by Sidak’s multiple comparisons test, *p < 0.05, **p < 0.01. d, e Lactyation and H3K18la levels were detected in OCM1 (d) and CRMM1 (e) cells cultured in different concentrations of 2-DG or oxamate for 24 h by Western blot. f Lactyation and H3K18la levels were detected in OCM1 and CRMM1 cells after LDHA and LDHB silencing by Western blot. g, h Proliferation of OCM1 (g) and CRMM1 (h) cells after LDHA and LDHB silencing was analyzed using CCK8 assay. **p < 0.01, ****p < 0.0001. i Tumor growth of OCM1 and CRMM1 cells after LDHA and LDHB silencing was evaluated by colony formation assay. j Statistical analysis of the colony formation assay performed using OCM1 and CRMM1 cells after LDHA and LDHB silencing. All of the experiments were performed in triplicate, and relative colony numbers are shown as means ± SD. *p < 0.05, ***p < 0.001. k The migratory ability of OCM1 and CRMM1 cells after LDHA and LDHB silencing was evaluated by transwell assay. l Statistical analysis of cells in the transwell assay performed using OCM1 and CRMM1 cells after LDHA and LDHB silencing. All of the experiments were performed in triplicate, and relative cell numbers are shown as means ± SD. *p < 0.05, ***p < 0.001