Fig. 1

Promoter, enhancer and gene expression mapping demonstrates consistent tissue-level gene regulation in mammals. a Example conserved, tissue-specific regulatory landscape around Myosin Heavy Chain 1 and 2 genes (Myh1 and Myh2) in muscle tissue from ten mammalian species. Inset numbers are maximum read depths for ChIP-seq and RNA-seq, while phylogenetic relationships and species divergences are shown on the left (see Additional file 1: Figure S1 for experimental workflow). b For the same locus as in a, regulatory landscapes in the liver, muscle, brain, and testis are shown for mouse and dog. c For each tissue, the number of biologically reproducible regulatory regions identified is consistent across species. Within a species, data is shown as stacked bar charts and the cross-species averages are similarly stacked below the graph. The average number of active promoters (purple), active enhancers (orange), and primed enhancers (green) across all species is shown below each column. The size of the underlying assemblies in Gigabases (Gb) is shown for all species. d The fraction of genes (diamonds) and transcripts (triangles) expressed in each tissue is stable across ten species. The average percentage of expressed genes across all species is shown as a dotted line with the confidence interval corresponding to +/− one standard deviation shaded. Species with larger differences between the fraction of expressed genes and transcripts have more comprehensive annotation