Fig. 4
From: Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding
Ribosome collisions occur at the amino acids that can terminate α-helices. a Volcano plots show the P-site pausing score (based on disome and monosome footprints, respectively) of each amino acid and the corresponding Q value (false discovery rate). Six amino acids with > 1 P-site pausing scores in disome-seq are labeled. P, proline; G, glycine; N, asparagine; K, lysine; C, cysteine; R, arginine. b The scatter plot shows the P-site pausing scores estimated from disome-seq or monosome-seq. The dashed line shows y = x. c The scatter plots show the P-site pausing score of an amino acid and the ratio of the whole-genome occurrence of the amino acid in gaps vs. in α-helices. The P value was given by linear regression. The dashed line represents the linear regression line (if statistically significant). The residuals of both linear models followed the normal distribution (P = 0.63 and P = 0.80, the Kolmogorov-Smirnov tests). d The scatter plots show the P-site pausing score of an amino acid and the propensity to terminate an α-helix by the amino acid (ΔG relative to alanine, in the unit of kJ/mol). The P value was given by linear regression. The dashed line represents the linear regression line (if statistically significant). The residuals of both linear models followed the normal distribution (P = 0.25 and P = 0.89, the Kolmogorov-Smirnov tests)