Fig. 5
Alteration of m6A in the mouse model of Alzheimer’s disease. a Normalized read density around 3′ UTR m6A peaks in WT and 5XFAD. There is less m6A read density in 5XFAD compared to WT. Input serve as background. b LC-MS/MS m6A quantification, showing significantly higher m6A levels in WT compared to 5XFAD. c IGV screenshots showing hypomethylation in FAD compared to WT in the 3′ UTR of four representative genes. d Top gene ontology biological processes and reactome pathway terms of genes that show hypomethylation in FAD compared to WT. e Scatter plot showing no change in mRNA levels of differentially methylated genes (shown in blue) between FAD and WT. f Cumulative frequency plot showing the correlation between differential methylation and protein levels. Differentially hypomethylated genes in FAD show a smaller log2 FAD/WT compared to all genes. This indicates that hypomethylated genes in FAD correlate with less protein levels. g RT-qPCR validation, showing no significant difference in mRNA levels for the four representative genes. The results were normalized to ß-actin house-keeping gene. h Western validation and quantification using ImageJ, showing higher levels of protein for those four representative genes in WT compared to 5XFAD