Fig. 6
Transcription-induced DNA damage in the absence of condensin. a Violin plots of lengths of constraint (Lc) in the indicated strains. ***p = 7.6 × 10−12 (WT vs cut14+), p = 1.3 × 10−51 (WT vs cut14SO), p = 2.6 × 10−16 (cut14+ vs cut14SO) (n > 300). b Image of cells with Rad52 foci in cut14SO. White arrowheads point to Rad52 foci. c Fraction of cells in the indicated conditions with Rad52 foci (five biological replicates, n ≥ 74); black bars show the median. n.s., no statistical significance; **p = 0.009, ***p = 0.0003. d Fraction of cut14+ and cut14SO cells with Rad52 foci following control or thiolutin (Th) treatment (three biological replicates, n = 200). Black bars show the median. n.s., no statistical significance; *p = 0.013, ***p = 0.005. e DNA volumes were quantified by DAPI staining as in Fig. 1d. Boxplots show DNA volumes in cut14+ and cut14SO cells with or without thiolutin (Th) treatment. Data from three biological replicates (n > 50), identified by their distinct shapes, is compiled into one boxplot. n.s., no statistical significance; ***p < 2.53 × 10−8