Fig. 1
Comparative transcriptional analysis reveals tumor-reactive CD8+ T cells to have a TRM signature with high expression of exhaustion markers. a Experimental design for the isolation of different CD8+ T cell populations from CRC patients. b, c Representative plots of FACS-isolated T cell populations. d Gene expression heat map of five CD8+ T cell populations. Rows represent signature genes, and columns represent cell types. Selective specifically expressed genes are marked in red. e GSVA was performed to identify enriched significant biological pathways in five CD8+ T cell subtypes. Five gene sets for each T cell population are depicted in a heat map. f PCA analysis of transcriptome expression of five CD8+ T cell populations. Each symbol represents one patient. g Volcano plot showing differential gene expression of CD103+CD39+ T cells vs. CD103−CD39− T cells (log2-transformed). Each red dot denotes an individual gene with a false-discovery rate (FDR) < 0.05. h Enrichment plot for the gene sets of “T cell exhaustion” and “TRM” in the transcriptome of CD103+CD39+ T cells vs. that of CD103−CD39− T cells by GSEA. NES, normalized enrichment score