Fig. 4

Fragile and stable enhancers employ different regulatory codes. a Different cohorts of TFBSs are enriched in enhancers with different densities of deMPs. The legend shows the range of fold enrichment (see the “Methods” section) of TFBSs in a set of enhancers relative to their negative control. b TFBSs in fragile enhancers are prone to one single-nucleotide deactivation, while TFBSs in stable enhancers are impervious to one single-nucleotide deactivation. c Homotypic TFBS clusters are more enriched in stable enhancers, whereas heterotypic TFBS clusters are more enriched in fragile enhancers. Only the TFBSs shown in Fig. 4a were included in the analysis. d Functional homotypic TFBS clusters are more enriched in stable enhancers, whereas functional heterotypic TFBS clusters are more enriched in fragile enhancers. Only the TFBSs located within corresponding ChIP-seq peaks and shown in a were included in the analysis. e Liver-specific TFBSs are more likely to form homotypic TFBS clusters (HTCs) in stable enhancers as compared to fragile enhancers. The asterisk indicates the Fisher exact test p value < 0.01