Fig. 3
De novo production of functional ZAM-derived piRNAs in the germline of the RevI-H2 line. a Pie chart summarizing the proportion of ZAM-derived piRNAs (allowing up to 3 mismatches) that map to the 142 piRNA clusters in wIR6 (no mismatch allowed, piRNA clusters defined as in [9]). b Density profile of ZAM-derived piRNAs along the 8.4 kb ZAM sequence in wIR6 ovaries (allowing up to 3 mismatches). Sense and antisense reads are represented in black and grey, respectively. ZAM organization is displayed above the profile. LTR, long terminal repeats. c Logo of nucleotide bias for the first ten positions in ZAM-derived piRNAs produced in wIR6 ovaries. The nucleotide height represents its relative frequency at that position. d Density profile of ZAM-derived piRNAs along the ZAM sequence produced in RevI-H2 ovaries (allowing up to 3 mismatches). Sense and antisense reads are represented in black and grey, respectively. e Bar diagram showing the total amount of ZAM-derived piRNAs produced in wIR6 and RevI-H2 ovaries, quantified from the profiles shown in b and c, respectively. f Logo of nucleotide bias for the first ten positions of ZAM-derived piRNAs produced in RevI-H2 ovaries. g Histogram showing the percentage of 5′-overlap between sense and antisense ZAM-derived piRNAs (23–29 nt) in wIR6 (top) and RevI-H2 (bottom) ovaries. The proportion of 10-nt overlapping pairs is in red, and the Z-score is indicated. h Bar diagram indicating the percentage of ZAM-derived piRNAs with ping-pong partners (PPP) in the wIR6 and RevI-H2 lines. i Analysis of nucleotide bias for sense (+) and antisense (−) ZAM-derived piRNAs with PPP in RevI-H2 ovaries. The percentage of PPP with a uridine at position 1 (1 U) and with an adenosine at position 10 (10A) is shown. j Confocal images of ovarioles after GFP (green, left panels) and DNA (blue, middle panels) staining. Ovarioles were from the progeny of a cross between wIR6 or RevI-H2 females and males carrying the pGFP-ZAM sensor transgene driven by actin-Gal4. Right panels, merged images of GFP and DNA labeling