Fig. 4

Regulatory networks inferred from 11 organs of the mouse body. a Marginal overlap of genes of different centralities in the brain network (top 20% genes). Additional organs in Additional file 1: Figure S5. b Visual representation of the mammary gland network, in which node size is proportional to pagerank centrality. The top five central nodes (red color) are all genes classified by OGEE as biologically essential. c Genes (2403) of the mammary gland regulatory network sorted by centrality. Increasing centrality corresponds to higher biological essentiality for all centrality measures. d Genes sorted according to their pagerank centrality. High centrality (roughly top 10% of genes) corresponds to high biological essentiality. e The central genes (here, pagerank, top 20%) of each organ classified by their multiplicity. Multiplicity = 1 means that they are central only in that organ, whereas multiplicity = 2(3+) means that they are also central in additional organs (total of 2, or 3 or more, organs). Additional organs in Additional file 1: Figure S3B. f Analysis of genes which are (i) central in at least one organ (pagerank) and (ii) upregulated in one organ compared to others. Intriguingly, most of the genes central in a given organ are actually expressed to a significantly higher extent (p < 0.05) in a different organ. Additional organs in Additional file 1: Figure S3D. g Radar plot of the pagerank ES score for the central genes of each organ (top 20%), partitioned in either organ-specific or shared (multiplicity 3+). The latter have higher biological essentiality and reach a significant p value for all organs (random permutations, p < 0.005 Additional file 1: Figure S3C). h Heatmap of GO enrichments using degree centrality