Fig. 4
From: CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
GEX1A treatment inhibits pre-mRNA splicing in WT rice but not the SGR4 mutant. The cDNAs were prepared from 1-week-old rice seedlings that were treated with 0.3 μM GEX1A for 6 h. RT-PCR was performed using primers that flank alternatively spliced introns in selected genes. Arrowheads indicate splicing variants that changed following GEX1A treatment. The gene structure flanking the amplified fragments and the structures of regulated variants are shown under the gel images (the green line marks the positions of the PCR product). Blue box, exon; line, intron; white box, 5′ or 3′ UTR. The gene locus identifier is shown on the bottom