Fig. 6From: Conbase: a software for unsupervised discovery of clonal somatic mutations in single cells through read phasinga Genotype predictions by Conbase in 11 sSNV sites. Primers spanning sSNVs and gSNVs were designed against these sites for PCR screening and Sanger sequencing. b, c Sanger sequencing results for the selected sSNV sites in amplified DNA from single CD8+ T cells identified by TCR sequencing as belonging to clones A, B, or an unrelated clone CBack to article page