Fig. 2
From: NmeCas9 is an intrinsically high-fidelity genome-editing platform
Characterization of functional PAM sequences in human (HEK293T) cells. a Split-GFP activity profile of NmeCas9 cleavage with ps9 sgRNA, with the target site flanked by different PAM sequences. Bars represent mean values ± s.e.m. from three independent biological replicates performed on different days. b T7E1 analysis of editing efficiencies at seven genomic sites flanked by PAM variants, as indicated. Products resulting from NmeCas9 genome editing are denoted by the red dots. c Editing efficiencies for chromosomal target sites with different PAM variants, as in B and C, as measured by deep sequencing analysis. d Quantitation of data from (b), as well as an additional site (NTS31; N4GACA PAM) that was not successfully edited. Bars represent mean values ± s.e.m. from three independent biological replicates performed on different days