Table 1 Statistics for the scaffold split regions tested by PCR
Statistics | Saker falcon | Ostrich | Budgerigar |
|---|---|---|---|
Pair-end read physical coverage within tested scaffolds | 135–524 | 2–604 | 0–631 |
No. split SF adjacencies by RACA (default param.) | 61 | 69 | 154 |
No. tested scaffold split regions | 22 (100%) | 49 (100%) | 43 (100%) |
No. amplified split regions (confirmed SF joints) | 11 (50%) | 32 (65%) | 20 (46%) |
No. non-amplified split regions | 11 (50%) | 17 (35%) | 23 (54%) |
No. tested RACA-suggested adjacencies | 11 | 8 | 18 |
No. amplified adjacencies (chimeric SF joints) | 5 | 7 | 11 |
Final no. ambiguous SF joints from tested split regions | 6 | 10 | 12 |
Selected pair-end read spanning threshold | 379 | 239 | 216 |