Fig. 3

Gene expression levels and editing activity of CRISPR/Cas9 in RNA-silencing pathway mutants. a A pipeline used to generate F1 hybrids of CRISPR/Cas9 and GUUS lines in different mutant backgrounds, ago1-27 is shown as an example. b Northern blot showing sgRNA, miR167, and Cas9 transcript levels in RNA-silencing pathway mutants. c Relative integrity (RI) of the GUUS reporter gene in different samples was detected by real-time PCR. Each column indicates means ± SD of three technical replicates. For each mutant background, eight individuals were analyzed as biological replicates. **Indicates significant difference at p < 0.01 under two-tailed Student’s t test