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Fig. 1 | Genome Biology

Fig. 1

From: Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome

Fig. 1

Gydle assembly (top tracks) aligned to the IWGSC RefSeq v1.0 chromosome 7A pseudomolecule (bottom tracks, see [1]) at positions 14.5 - 17.2 Mb. The top two tracks show BAC pools 7AS-11848, 7AS-11877 and 7AS-00257 aligned to Bionano maps 7AS_0072 and 7AS_0036. The BAC pool assemblies are finished with no gaps or ambiguities and have resolved repeat arrays which are collapsed in the IWGSC RefSeq v1.0 assembly. Depending on the coverage of BACs, regions of the IWGSC RefSeq v1.0 assembly are either covered by a single BAC pool, covered by multiple BAC pools (such as the 30 Kb of overlap between 7AS-11848 and 7AS-11877) or not covered by any BAC pool (such as between 7AS-11877 and 7AS-00257). The Gydle assembly increased the assembled sequence length by a total of 169 Kb across the region covered by these three pools (approximately 8%)

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Genome Biology

ISSN: 1474-760X

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