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Fig. 6 | Genome Biology

Fig. 6

From: HmmUFOtu: An HMM and phylogenetic placement based ultra-fast taxonomic assignment and OTU picking tool for microbiome amplicon sequencing studies

Fig. 6

Comparing the quality of rep-seqs between HmmUFOtu’s consensus-based and QIIME’s single-sequence based rep-seq picking methods. For HmmUFOtu: the consensus sequences with (default) or without the priors were tested; for QIIME: the “first,” “longest,” and “random” methods were tested. a Mock datasets, in which the quality is reflected by the %identity between the rep-seqs and the known bacterial reference genomes. b HMP datasets, in which the quality is reflected by the %identity between the rep-seqs and the de novo assembled scaffolds from the WGS data sequenced in the same samples. LAH left auriculotemporal part of head, RAH right auriculotemporal part of head

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