Fig. 6

Gene disruption efficiencies in the presence of different single-stranded DNA donors. a, c Extent of indel formation at the a CACNA1D or c PPP1R12C genomic locus when various ssODN donor templates were used in combination with SpCas9, AsCpf1, or LbCpf1. The rates were quantified by Illumina deep sequencing. NT and T indicate donors of non-target and target strand sequences, respectively. Data represent mean ± standard error of the mean (n ≥ 4). *P < 0.05, **P < 0.01, ***P < 0.001; Student’s t-test. b, d Boxplot summarizing the rates of indel formation at the b CACNA1D or d PPP1R12C locus for oligonucleotides of different lengths and structures. There was no significant difference in the indel frequencies between NT and T ssODN donors and hence their data were pooled