Fig. 3

a Orthogonal partial least squares discriminant analysis (OPLS-DA) of individuals with (blue) and without (green) hepatic steatosis as assessed by fatty liver index. b Permutation validation of the plot in (a). R² and Q² values to the left of the plot are from random models compared with the values for the true model on the right. c S-plot of discriminatory metabolites in (a). Metabolites are coloured according to lipid class. d OPLS-DA of individuals with (yellow) and without (green) hepatic steatosis as assessed by ultrasound. e Permutation validation of the plot in (d). R² and Q² values to the left of the plot are from random models compared with the values for the true model on the right. f S-plot of discriminatory metabolites in (d). Metabolites are coloured according to lipid class. CE cholesterol ester, DG diacylglycerol, lyso-PC lyso-phosphatidylcholine, PC phosphatidylcholine, PE phosphatidylethanolamine, TAG triacylglycerol, SM sphingomyelin. g OPLS-DA scores plot of the TAG profile of individuals with (yellow) and without (green) hepatic steatosis as assessed by ultrasound. h S-plot of discriminatory TAGs in (g)