Fig. 1
From: Ythdf2-mediated m6A mRNA clearance modulates neural development in mice
Ythdf2−/− mice are embryonic lethal. a The gene-targeting strategy to disrupt the Ythdf2 gene in mouse. Conditional Ythdf2 gene-targeted mouse contains LoxP sites flanking the 5′ UTR and exon 1 of the endogenous Ythdf2 locus. WT_F wild-type forward primer, WT_R wild-type reverse primer, KO_F Ythdf2−/− forward primer, WT_R Ythdf2−/− reverse primer, Ex exon. b Numbers of offspring from heterozygous Ythdf2+/− intercrosses. The number and genotype of embryos at E12.5/E14.5 and postnatal are indicated. c PCR analysis of embryo tail DNA showing a 271-bp wild-type band (WT) and a 550-bp targeted band (KO) with primers displayed in a. d Western blot analysis of the Ythdf2 expression in wild-type and Ythdf2−/− embryos. Two samples for each genotype. Actin was used as loading control. e Numbers of embryos per litter at E12.5/E14.5 and E18.5 from wild-type or heterozygous intercrosses. Error bars represent mean ± standard deviation, n = 7 litters. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test